Fig. 2
From: An HDAC3-PROX1 corepressor module acts on HNF4α to control hepatic triglycerides
HDAC3 and PROX1 exhibit extensive co-binding and reveal a metabolic signature. a Venn diagram displaying overlap of peaks identified in PROX1 (2 rpm cutoff) and HDAC3 (1.5 rpm cutoff) ChIP-seq. Peaks required 50% overlap and have a minimum 1 rpm signal for the other factor. b Representative browser tracks of HDAC3 and PROX1 ChIP-seq. Scale is reads per ten million (RPTM). c Co-occupancy of PROX1 and HDAC3 as indicated by ChIP–reChIP (n = 3) from liver. Legend indicates reChIP antibody following primary PROX1 ChIP elution. Data are presented as mean ± s.d., one-tailed unpaired Student’s t-test, *P < 0.05, **P < 0.01, ***P < 0.001, ns not significant. d Reactome analysis of the nearest genes within 100 kb from the top 1000 overlapping HDAC3 and PROX1 peaks. e HOMER motif analysis of co-bound peaks displaying over-represented sequences. f HOMER motif enrichment analysis of the indicated motifs (HNF4α and Rev-erbα DR2) at overlapping and non-overlapping peaks determined in (a). Numbers above brackets indicate P-values, χ 2 test
