Fig. 4
From: MicroRNA-9 downregulates the ANO1 chloride channel and contributes to cystic fibrosis lung pathology
miR-9-specific TSB increases ANO1 expression, chloride activity, and migration rate. CF cells (CFBE41o-) were transfected with TSB control (control) or ANO1 TSB for 24 h. a ANO1 protein expression was analyzed and quantified by western blotting using anti-ANO1 antibody. β-actin was used for normalization. (n = 4, in triplicates). Histograms represent average values ± SDs and were compared using Student’s t-test. b Kinetics of YFP-H148Q/I152L protein quenching after addition of I− to the medium. Twenty-four hours after transfection with YFP-H148Q/I152L plasmid, cells were selectively microinjected with TSB control or ANO1 TSB as indicated. Scale bar 5 µm. c Representative and original traces of ANO1 channel activity (left) and quantification (right) of CF bronchial epithelial cells transfected with ANO1 TSB or a negative control (n = 8, in triplicates) as compared to non-CF cells (16HBE14o-). Histograms represent average values ± SDs and the conditions were compared using ANOVA coupled with Dunnett’s, Bonferroni’s and Tukey’s post hoc test. d Migration rates during repair of CF cells. Representative images were taken during 4 h of wound closure of CF cells (left) and migration rates were quantification (right) (n = 5). Scale bar 10 µm. Histograms represent average values ± SDs and were compared using Student’s t-test
