Fig. 6
From: MicroRNA-9 downregulates the ANO1 chloride channel and contributes to cystic fibrosis lung pathology
miR-9-specific TSB is well tolerated in CF mice. For all the experiments, we used 8-week-old male 129-Cftr tm1Eur CF model mice homozygous for the F508del mutation in the 129/FVB outbred background (F508del-CFTR) and their wild-type littermates obtained from CDTA-CNRS (Orléans, France). a Representative and original traces of ANO1 channel activity (left) and quantification (right) of MLE15 cells transfected with TSB control (control) or ANO1 TSB. Histograms represent average values ± SDs (n = 5) and were compared using Student’s t-test. b Outline of the CF mouse experiment. TSB control or ANO1 TSB was instilled intranasally at days 7 and 14 after reception (day 0), and mice were killed at day 21. c Growth curves of mice from the day of reception (day 0). Arrows represent intranasal instillation
