Fig. 7

miR-9-specific TSB increases ANO1 activity and mucus dynamics in CF mice. For all the experiments, we used 8-week-old male 129-Cftr ^tm1Eur CF model mice homozygous for the F508del mutation in the 129/FVB outbred background (F508del-CFTR) and their wild-type littermates obtained from CDTA-CNRS (Orléans, France). a Representative and original traces of ANO1 channel activity (left) and quantification (right) of cells isolated from wild-type mice (n = 8), CF mice instilled with ANO1 TSB (n = 7) or a negative control (n = 7). Histograms represent the average values ± SDs and were compared using one-ANOVA test coupled with Dunnett’s, Bonferroni’s, and Tukey’s post hoc test. b Effect of TSB control or TSB ANO1 on the transport of phenol red dye in the trachea. Histograms represent the average values ± SDs and were compared using one-way ANOVA test coupled with Dunnett’s, Bonferroni’s, and Tukey’s post hoc test. c Effect of TSB control or ANO1 TSB on mucus dynamics on the trachea of CF mice. The movements of 100 beads were quantified for each condition, and the average speed (µm/ms) was determined. Scale bar 40 µm. Histograms represent the mean values ± SDs and were compared using Student’s t-test