Fig. 1
From: Resonant out-of-phase fluorescence microscopy and remote imaging overcome spectral limitations

Principle of speed OPIOM. a A sinusoidally modulated antiphase-synchronized dual illumination generates the quadrature-delayed component S (in red) of the fluorescence emission used for selective speed OPIOM imaging of RSFPs. b After in situ calibration, the fluorescence images are recorded under modulated illumination and processed to yield S after Fourier transform