Fig. 3
From: Resonant out-of-phase fluorescence microscopy and remote imaging overcome spectral limitations
Speed OPIOM selectively retrieves an RSFP signal in the presence of spectral interferences. In contrast to pre-OPIOM (b, e), speed OPIOM microscopy (a) and remote (d) imaging selectively unveils a RSFP target (square in a, d, Dronpa-2 in c–f) in cells (a) and Camelina seedlings (d) even in the presence of a spectrally interfering fluorophore (disk in a, d, EGFP in b, c), autofluorescence or ambient light (d) and (e, f). Systems: Fixed HeLa cells expressing H2B-Dronpa-2 (at the nucleus) and Lyn11-EGFP (at the cell membrane). b, c Camelina, ubiquitously expressing Dronpa-2 or wild type (respectively top and down in e, f). The images were recorded at 37 °C (b, c) and 20 °C (e, f). Scale bars (µm): 10 (b, c), 875 (e, f). See Supplementary Tables 1 and 3 for the acquisition conditions
