Fig. 5
From: Resonant out-of-phase fluorescence microscopy and remote imaging overcome spectral limitations
Speed OPIOM can track quantitatively fast biological processes. Live HeLa cells expressing MEK1, Kohinoor-ERK2 (initially located in the cytoplasm) and Lyn11-Dronpa-3 before (a–c) and after (d) addition of epidermal growth factor (EGF). The figure in e displays the temporal evolution of the absolute value of the ratio ρ of the speed OPIOM Kohinoor signals in the nucleus and in the cytoplasm. Pre-OPIOM and speed OPIOM images tuned to the resonance of Dronpa-3 and Kohinoor (a–e). Localizations: Lyn11 (cell membrane). The images were recorded at 37 °C. Scale bars: 20 µm. See Supplementary Table 1 for the acquisition conditions
