Fig. 6
From: Resonant out-of-phase fluorescence microscopy and remote imaging overcome spectral limitations
Speed OPIOM can independently image four spectrally similar fluorescent proteins without high-contrast enhancement. Pre-OPIOM (a) and speed OPIOM images tuned to selectively image Dronpa-2 (b), Padron (c), and Dronpa (d). In e, overlay between the pre-OPIOM image from the Dronpa-2 acquisition and speed OPIOM images collected in (b–d). Systems: Fixed U2OS cells expressing H2B-Dronpa-2, Mito-Padron, Dronpa-GTS, and Lyn11-EGFP. Localizations: H2B (nucleus), Lyn11 (cell membrane), Mito (mitochondria), GTS (Golgi). The images were recorded at 37 °C. Scale bars: 20 µm. See Supplementary Table 1 for the acquisition conditions
