Fig. 4

Volumetric imaging of spatially distributed carbon fibers. a Volume-rendered image of a 3D object reconstructed by SIR-PAM, showing spatially invariant resolution. b Image of the same region as in a obtained by conventional PAM with decreasing lateral resolution away from the focal plane. Three pairs of en face image slices were taken at imaging depths of –0.7, 0.0, and 0.8 mm. The focal plane is located at z = 0.0 mm. c−e Corresponding line profiles across the carbon fibers shown to compare the imaging quality of the two systems. Scale bars, 150 µm