Fig. 4

Alternative NF-κB signalling is stimulated by TRAF3 autophagy. a A549 cells were transfected with the indicated siRNA for 72 h and immunoblotted as shown (p100 and p52 indicate parental and processed NFκB2, respectively). b, c A549 WT, ΔATG5 and/or rescue cell clones were immunoblotted as shown. d A549-Cas9 control cells or pooled ΔFIP200 counterparts were immunoblotted as shown. e A549-Cas9 control cells or pooled ΔFIP200 and ΔNDP52 counterparts were subcutaneously injected into immunocompromised mice and tumour volume was measured longitudinally (means, n = 8 flanks, ±S.E.M., *P < 0.05 vs. A549-Cas9, two-tailed t-test). f NCI H23-Cas9 control cells or pooled ΔATG5 counterparts were immunoblotted as shown. g, h Wild-type (WT) or Atg5 null (Atg5−/−) empty vector (EV) or RAS-transformed (KRAS V12) MEFs were g stained for RELB or h immunoblotted. In g, wide-field images are shown (scale bar = 20 µm) and cells with nuclear RELB quantified (means, n = 3, ±S.E.M., **P < 0.01, ^# P > 0.05, ANOVA with Tukey’s post hoc test). i WT or Atg5−/− MEF KRAS-V12 cells were transfected with the indicated siRNA for 32 h and then immunoblotted. Uncropped blots are available in Supplementary Fig. 10