Fig. 2
Response of NiV G mutants to ephrinB2-Fc binding measured by SHG and conformational antibody binding. a Change in SHG signal of NiVG ectodomain constructs bound at 0.5 μM at 20 min following ephrinB2-Fc addition to 0.6 μM. b Binding affinity of ephrinB2-Fc to NiV G ectodomain constructs measured by direct ELISA. c Inhibition of wildtype NiV G binding to ephrinB2-Fc by wildtype NiV G and a C387A mutant. d Binding of mAb213 to NiV G constructs with increasing ephrinB2-Fc concentration. e Binding of mAb45 to NiV G constructs with increasing ephrinB2-Fc concentration. f Binding of mAb45 to wildtype NiV G and a C387A mutant with increasing ephrinB2-Fc concentration. Mean and s.d. values for SHG data are shown from a representative experiment, n = 3. Mean and s.d. values for ELISA data are shown from a representative experiment, n = 2
