Fig. 2

Zfp36 ^−/− CD8⁺ T-cells have no intrinsic defect in IFN-γ production. a Naive CD8 T-cells from WT and Zfp36 ^−/− mice were stimulated by plate-coated α-CD3/CD28 Abs (1 μg/ml). CD69, CD25, CD44 and CD62L surface expression were detected by FACS at different time points as indicated. b, c Quantitative data represent means ± s.d. of numbers b and percentages c of CD69⁺, CD25⁺, CD44⁺ and CD62L⁺ CD8 T-cells as in a from three independent experiments. d Purified naive CD8 T-cells from WT and Zfp36 ^−/− mice were stimulated by plate-coated α-CD3/CD28 Abs (1 μg/ml) for 72 h. Then the expression of IFN-γ, T-bet, Eomes, TNF, Blimp-1 and ZEB2 mRNAs were detected by qRT-PCR. The qRT-PCR data were normalized relative to GAPDH mRNA levels and further normalized to the results from WT group. Results of three independent experiments shown are means ± s.e.m. e IFN-γ and TNF levels in cell supernatants as in a were detected by ELISA. Quantitative data shown are means ± s.d. from three independent experiments. f Naive CD8⁺ T-cells from WT and Zfp36 ^−/− mice were stimulated by plate-coated α-CD3/CD28 Abs (1 μg/ml) for 3 days, rested for 3 days, and then stimulated by PMA and Ionomycin in the presence of GolGistop for 4 h. IFN-γ was detected by FACS gated on CD8 T-cells. Quantitative data shown are means ± s.d. from three independent experiments. Data are analyzed with unpaired students’t-test as in b–f, *p < 0.05