Fig. 2 | Nature Communications

Fig. 2

From: Bone corticalization requires local SOCS3 activity and is promoted by androgen action via interleukin-6

Fig. 2

High bone formation in 6-week-old male and female Dmp1Cre.Socs3 f/f mice. a–c Histomorphometric analysis of osteoblast surface (a), osteoid surface (b) and osteoclast surface (c) in the secondary spongiosa of 6-week-old male and female Dmp1Cre.Socs3 f/f (gray squares) and Dmp1Cre (open circles) tibiae; values are mean ± SEM, n is shown below (a). *p < 0.05; **p < 0.01; ***p < 0.001; vs Dmp1Cre of the same sex by two-way ANOVA with Sidak post-hoc test. d Representative images of calcein labeling of bone (b) in male and female Dmp1Cre.Socs3 f/f and Dmp1Cre mice; m, marrow. Scale bar = 500 µm. e High-power images of the boxed regions in panel (b) showing lamellar calcein labels in the secondary spongiosa in female Dmp1Cre tibiae and woven-bone formation in the same region in female Dmp1Cre.Socs3 f/f mice. Scale bar = 50 µm. e Safranin O staining shows no elevation in cartilage remnants (orange) within the trabecular bone (b) of female Dmp1Cre.Socs3 f/f mice compared with Dmp1Cre mice; scale bar = 50 µm

Back to article page