Fig. 6

Histological analysis detected less immune response in MHC-matched transplantation. a Representative low magnified views of staining (Cont#4 and Hetero#4 from the HT1 experiments) at 4 months after transplantation. b Staining of Cont#5, Hetero#5, Cont#7, and Hetero#7 from the HT4 experiments. Representative view of grafts stained with DAB-Ni immunostaining for IgG, MHC-II, Iba-1, and CD45 are shown. c–f Magnified representative view of IgG deposits (c), Iba-1 (d), and CD45 (e) DAB-Ni staining in the grafts. Pictures are from Mismatch#4 (c–e) and Match#4 (c, d). f Representative view of immunostaining for CD3 (green) and CD4 (magenta) in the accumulation of lymphocytes in Mismatch#4. g, h Analyses of infiltrated lymphocytes. The majority of lymphocytes (CD3+) were positive for CD4 (helper T cell, green) and some were positive for CD8 (cytotoxic T cell, magenta; g). CD45 positive lymphocytes were derived from the host without GFP expression (h). i Immunostaining of the graft with GFP and Iba-1. j–p Activated natural killer (NK) cells stained by NKp46/NCR1 antibody (magenta). Low magnification (j–l), high magnification (m–o), and quantification (p). Scale bars: 2 mm (a), 5 mm (b; IgG), 100 μm (b; MHC-II, Iba-1, and CD45), and 50 μm (c–i, m–o), 500 μm (j–l). Quantitative data are presented as means ± SD (p)