Fig. 7
From: MHC matching improves engraftment of iPSC-derived neurons in non-human primates
Quantitative analysis showed the effect of MHC matching on decreasing immune response after transplantation. a–d Quantitative analyses of Iba-1+ microglia (a, b) and CD45+ leukocytes (c, d) in the grafts; HT1 (a, c) and HT4 (b, d) experiments. e, f Averaged value plots of Iba-1+ (e) and CD45+ (f) cell densities in the grafts of individual monkeys. Combined data of Mismatch and Match from the HT1 and the HT4 series. g–j Averaged value plots in individual monkeys for the expressions of Iba-1 (g), CD45 (h), MHC class II (i), IgG deposits (j), 11C-PK11195 (k). Mismatch (n = 6), Match (n = 6), Mismatch + Tacrolimus (n = 2), and Match + Tacrolimus (n = 2) experiments were analyzed. HT1 (#1–#4) and HT4 series (#5–#8) are separately shown in each graph. l The calculation method of optical density for MHC class II and IgG deposit staining. m Morphological analysis of Iba-1+ microglia. Resting microglia change their shape from ameboid to round upon activation. Data are presented as means ± SEM (n = 24 tracts for a, c; n = 12 tracts for b, d; n = 6 animals for e, f). Paired t-tests were performed for the HT1 experiment: ****P < 0.0001 (a), *P = 0.029 (c), **P = 0.004 (e), *P = 0.023 (f). One-way ANOVA with Tukey’s multiple comparisons test was performed for the HT4 experiment: ***P = 0.0005 (b), *P = 0.0496 (b), ***P < 0.0001 (d). Tac: Tacrolimus, See also Fig. 6
