Fig. 8

Histological analysis detected more dopamine neural survival in MHC-matched transplantation. a, b Representative low magnified views of staining at 4 months after transplantation. Cont#4 and Hetero#4 from the HT1 experiments (a). Cont#5, Hetero#5, Cont#7, and Hetero#7 from the HT4 experiments (b). Representative view of grafts with Hematoxylin and Eosin (HE) staining (a, b) and DAB-Ni immunostaining for tyrosine hydroxylase (TH; a, b) and Foxa2 (b). c–e TH (green), Foxa2 (c, magenta), and Girk2 (d, e, magenta) staining in the representative graft (Hetero#1). Magnified view (c, e) and lower magnified view (d). f, g Quantitative analyses of TH+ cells (f, g), Foxa2 (h, i), and graft volume (j, k) in the grafts; HT1 (f, h, j; n = 24 tracts) and HT4 (g, i, k; n = 12 tracts) experiments. l, m Averaged value plots of TH+ (g) and Foxa2+ (h) cell densities in the grafts of individual monkeys. Combined data of Mismatch and Match from the HT1 and the HT4 series. n–p Averaged value plots in individual monkeys for the expressions of tyrosine hydroxylase (TH, n), Foxa2 (o), and graft volume (p). Mismatch (n = 6), Match (n = 6), Mismatch + Tacrolimus (n = 2), and Match + Tacrolimus (n = 2). Scale bar: 5 mm (a HE and TH, b HE), 1 mm (b, TH lower mag), 100 μm (b TH and Foxa2, d), and 50 μm (c, e). Quantitative data are presented as means ± SEM (n = 24 tracts for f, h, j; n = 12 for g, i, k; and n = 6 for l, m). Paired t-tests were performed for f, h, j, l, m: **P = 0.004 (f) **P = 0.008 (l). One-way ANOVA with Tukey’s multiple comparisons test was performed for g, i, k: ****P < 0.0001 (g), ***P = 0.0001 (g), *P = 0.049 (g, left), *P = 0.022 (g, right), *P = 0.025 (i, upper), **P = 0.006 (i), *P = 0.022 (i, lower), *P = 0.024 (k). Tac: Tacrolimus