Fig. 8
From: The telomere binding protein Pot1 maintains haematopoietic stem cell activity with age
MTM-POT1a improves aged HSC function. a Schematic of the analysis of cell number and apoptotic fraction of 90 week-old LT-HSCs after culture with MTM-POT1a or control MTM protein. b Number of total cells and LT-HSCs on days 4, 7, and 10 of culture. Data are expressed as the mean ± SD (n = 5: day 4, n = 6: day 7 and 10, **p < 0.05 by t-test). c Percentage of Annexin V+PI+ apoptotic cells in LT-HSC fraction on day 4, 7, and 10 of culture. Data are expressed as the mean ± SD (n = 6–8). d, e Effect of MTM-POT1a on reconstitution after BMT of young and aged LT-HSCs. 8 and 90 week-old LT-HSCs were cultured with MTM-POT1a or control MTM protein for 10 days and transplanted into lethally irradiated mice. d Percentages of donor-derived (Ly5.1+) cells in PB and LT-HSCs 4 months after BMT are shown (n = 5 per group, *p < 0.01, **p < 0.05 by Tukey’s test). Representative data from two independent experiments are shown. e Percentage of T cells (CD3+), B cells (B220+), and myeloid (Mac-1+/Gr-1+) cells in donor-derived cells in PB (n = 5 group–1, *p < 0.01, **p < 0.05 by Tukey’s test). Representative data from 2 independent experiments are shown. f 60 week-old LT-HSCs were cultured with MTM-POT1a or control MTM protein for 3 days. After the culture, gene expression profiles were analyzed by microarray. GSEA plots demonstrating enrichment levels of indicated gene sets in control MTM protein treated cells versus MTM-POT1a treated cells. NES, NOM P value, and FDR are indicated
