Fig. 2
High-throughput SRS microscopy screens using photo-highlighting. a The methodological scheme of SRS-based forward genetic screens using photo-highlighting. b The working flow of primary screens and subsequent retests to identify mutants with altered lipid distribution phenotypes. c, d 57 mutants were identified with altered lipid distribution and categorized into five classes. Age synchronized, 1-day-old adult worm were imaged. Example SRS images are shown in c. Dashed lines indicate the boundary of animals. High laser power: 600 mW pump/OPO (816 nm), 400 mW Stokes/IR (1064 nm); low laser power: 200 mW pump/OPO (816 nm), 400 mW Stokes/pump (1064 nm). Scale bar = 50 μm; Hyp, hypodermis; Int, intestine; LD, lipid droplets; Ooc, oocytes. e Example, high-resolution images show enlarged lipid droplets in the intestine of class II mutants. Scale bar = 50 μm
