Fig. 2
From: Molecular identification of the wheat male fertility gene Ms1 and its prospects for hybrid breeding
TaMs1 confers male fertility and is expressed in developing wheat anthers. a Scanning electron micrographs showing the defects in tapetal cell surface-localised orbicule (Or) structures and pollen coat (inset) within male sterile (ms1d) vs. wild-type anthers (Ms1). Scale bars: 2 µm (inset 10 µm). b TaMs1 and homeologue mRNA levels as detected by qRT-PCR in premeiotic to mature pollen-containing anthers, leaf/shoot apical meristem and roots. The data are means ± s.e.m (n = 3 biological replicates). c Histochemical GUS analysis of wheat anthers expressing a translational GUS fusion with TaMs1 (native promoter). Transverse section of a wheat anther containing microspores undergoing meiosis showing cell-type-specific GUS expression. Ruthenium red-stained cell walls (pink). Scale bar: 50 µm. (Ta Tapetal cell, M Microspore)
