Fig. 1
From: Blood vessel control of macrophage maturation promotes arteriogenesis in ischemia
Origin of macrophages in ischemia. a Representative flow cytometric analysis of ischemic tibialis anterior muscle of Cx3cr1 GFP/+ mice. Granulocytes (green), Ly6Chi monocytes (blue) and macrophages (black), n = 3 mice/group. b Adoptive cell transfer at d1 after HLI and cell fate tracking of CD45.2+CD11b+GFP+Ly6Chi monocytes transferred into CD45.1+ recipients. Representative flow cytometry of recipient muscle, replicated at least three times. c Treatment with CCR2 blocking antibody MC-21 (α) or isotype control c for indicated time intervals (r × d) after HLI and analysis of cell populations in peripheral blood (PB) and muscle (M) by flow cytometry. n = 5/7/7 mice/group, error bars represent s.e.m. ***p < 0.001, **p < 0.01, *p < 0.05 *indicates comparison between treatment with MC-21 (α) vs isotype control c by one way ANOVA with Bonferroni multiple comparison test. d Quantitative RT-PCR analysis of cell populations of Cx3cr1 GFP/+ mice sorted from d3 ischemic muscle relative to bone marrow (BM) Ly6Chi monocytes. n = 3 independent experiments, error bars represent s.e.m. ***p < 0.001, **p < 0.01, *p < 0.05, #Comparison between muscle and BM Ly6Chi cells, *Comparison between muscle Ly6Chi and muscle macrophages (MF), one way ANOVA with Bonferroni multiple comparison post-test
