Fig. 6

Canonical Notch signaling regulates macrophage maturation in vivo. a Analysis of cell populations by flow cytometry in ischemic muscle of Rbpj conditional mutant mice (Rbpj ^ΔM) and control mice. n = 7/9 mice/group, error bars represent s.e.m., *p < 0.05 by two way ANOVA with Bonferroni post test. b Representative flow cytometry of 6 h in vivo BrdU incorporation in macrophages from ischemic muscle at d3. n = 3 independent experiments c Representative flow cytometry of macrophages (CD11b⁺, F4/80⁺) from ischemic muscle at d3. n = 4 independent experiments d Gene expression analysis of macrophages sorted from d3 ischemic muscle. n = 3 independent experiments, error bars represent s.e.m. ***p < 0.001, **p < 0.01, *p < 0.05, unpaired Student’s t-test. e Representative cell death analysis in macrophages (CD11b⁺, F4/80⁺) from ischemic muscle. n = 4 independent experiments. f Gene expression analysis of isolated Ly6C^hi monocytes from wild type or Rbpj ^∆M mice (0) and derived macrophages cultured on DLL1 for indicated time. n = 3 independent experiments measured in duplicates, error bars represent s.e.m. ***p < 0.001, **p < 0.01, two-way ANOVA and Bonferroni post-test. g Representative BrdU incorporation of d7 macrophages on DLL1. n = 4 independent experiments