Fig. 1

Schematic of sample isolation and next-generation sequencing analyses. (Top panel) Tumor sites analyzed from CGOV62 with stage III HGSOC. For each sample, slides were stained with hematoxylin and eosin as well as analyzed by immunohistochemical staining of p53. (Middle panel) Tumor samples were microdissected for genomic analyses. For microdissection for STIC and p53 signature lesions, tumor cells were identified using immunohistochemical staining of p53 and isolated through laser capture microdissection. (Bottom panel, left) Next-generation sequencing analyses were performed for tumor specimens using either whole-exome or targeted analyses. (Bottom panel, right) Somatic mutations and chromosomal alterations were used to evaluate tumor evolution using the tumor subclonality phylogenetic reconstruction algorithm SCHISM and to determine a timeline for tumor progression