Fig. 2
Chromosomal fragment deletions identified in inducer CAU5 pollen. a Bright illumination plus fluorescence shows pollen cut with the tip of a glass micropipette. b One putative trophic nucleus and two putative sperm visualized by fluorescence. In a, b, DNA is visualized by SYTOX Green fluorescence (bar = 100 μm). c–g Pollen grains 1–5 have only one or two defective chromosomes. Some fragments were deleted in one sperm (shown on light green background) or not segregated (deletion in one sperm and diploidy in another sperm, shown on tan color background). h Pollen 6 containing two sperm with a large-scale deletions in almost every chromosome. The darker green background shows the deletion regions that occurred in the two putative sperm. In c–h, blue and red spots represent CNVs in odd and even chromosomes, respectively; the three data sets are results of putative trophic nucleus, putative sperm 1, and putative sperm 2, from top to bottom; the black, blue, and red lines around spots indicate normal haploidy, deletion, and diploidy, respectively. The black triangles point to centromeres
