Fig. 5 | Nature Communications

Fig. 5

From: Single nucleus sequencing reveals spermatid chromosome fragmentation as a possible cause of maize haploid induction

Fig. 5

A model for haploid generation. Two classes of aneuploid pollen grains were identified. In theory, both classes should carry one aneuploid sperm or two aneuploid sperm, and fertilize egg and/or polar nucleus. Class I pollen grains could induce haploid embryos, genome elimination endosperms, and kernels with approximately equal frequency. Class II pollen grains can fertilize egg and/or polar nucleus and may generate normal or abnormal kernels with a few chromosome fragments lost but not haploid kernels. The frequency of class I is higher than class II based on the present data. Eight haploids with paternal genome elimination were detected in 81 embryo samples. However, no paternal genome elimination was detected in 81 endosperm samples. It is possible that Class I endosperm with genome elimination causes abnormal kernels and may have been ignored when sampling

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