Fig. 5
Comparison of Ch-CPN with Group II CPN. a Superposition of subunit structures of Ch-CPN (cyan) and Group II TKS1-CPN (PDB entry: 1Q3S31, orange). Significantly different regions between Group II and III CPNs are indicated by dotted circles. b Top views of the lid of Group II (right) and Group III CPNs (left). c Close-up views of the lid (left) and nucleotide-sensing loop (right) regions of Group II and III CPNs. The lid of TKS1-CPN (orange) is rotated 45° relative to Ch-CPN (cyan). In the nucleotide-sensing loop, Arg155 of Group III CPN (cyan) is equivalent to Lys165 (orange) in Group II. Bound AMPPNP is shown in a gray stick. d Structure-based sequence alignments of the lid (upper) and nucleotide-sensing loop (bottom) in Group II and Group III CPNs. Deletions in the lid of Group III CPNs are indicated by a green box (upper). Residues constituting nucleotide-sensing loops are highlighted in yellow backgrounds (bottom)
