Fig. 6
From: Supramammillary glutamate neurons are a key node of the arousal system
Activation of SuMvgat neurons results in brief wakefulness. a Cartoon showing AAVx-FLEX-hM3Dq-mCherry recombining in vgat neurons that express Cre. b An example injection site showing hM3Dq transduction (red outline) in the region containing GABA-glutamate neurons in the SuMg (lower left—duration of wakefulness after CNO in this mouse, scale bar 500 µm). c, d Hypnograms showing sleep−wake in two mice with saline, then CNO, with a transient increase in wakefulness. e, f Example CSA’s corresponding to the periods shown by the blue and red boxes in c and d, respectively (0−100 Hz, black bar shows the dark period, short white bars represent 20 min and are spaced 1 h apart)
