Fig. 4
From: DNA-binding of the Tet-transactivator curtails antigen-induced lymphocyte activation in mice
The tTA transactivator induced loss of germinal centre and splenic plasma cells in immunized mice is B cell-autonomous. Flow cytometric analysis of splenocytes from Eμ-tTA mice (n = 6) or littermate control (n = 6) at day 7 after immunization with NP-KLH. a Representative stainings with antibodies against B220 and CD138 markers to identify plasma cells (left) or NP-specific IgG1+ germinal centre B cells (left) defined as IgM− IgD− Gr1− CD138− B220+ IgG1-NP+. For the gating strategy, please see the Supplementary Fig. 9b. b Frequencies of germinal centre NP-specific isotype-switched B cells (IgM− IgD− Gr1− CD138− B220+ IgG1-NP+) and splenic plasma cells (B220low CD138+). Data are presented as box-and-whiskers diagram with median and interquartile range. **P ≤ 0.01, Eμ-tTA vs. WT, two-way ANOVA with Bonferroni correction for multiple comparisons. c Frequencies of total NP-specific IgG1-secreting cells in spleen as determined by ELISPOT assay. Each dot represents data from a single mouse as the mean of assays in replicate wells, with four mice in each group, and summarizes two independent experiments. Data are presented as scatter plots with mean. ***P < 0.001, Eμ-tTA vs. WT, two-sample Kolmogorov–Smirnov test
