Fig. 5
From: DNA-binding of the Tet-transactivator curtails antigen-induced lymphocyte activation in mice
Tet transactivator-mediated cytotoxicity in antigen stimulated B cells depends on DNA binding. a Representative staining (left) and frequencies (right) of flow cytometric analysis of splenic plasma cells (B220low CD138+) from vav-tTA (n = 10), CAG-rtTA (n = 8) or littermate control mice (n = 9) at day 7 after immunization with NP-KLH or vav-tTA mice (n = 5), CAG-rtTA mice (n = 9) or littermate control (n = 8) fed with doxycycline-containing food for 7 days prior and during the 7 days of NP-KLH challenge. b Representative FACS analysis (left) and frequencies (right) of germinal centre NP-specific isotype-switched B cells (IgM− IgD− Gr1− CD138− B220+ IgG1-NP+) from the mice of the indicated genotype as in a. For the gating strategy, please see the Supplementary Fig. 9b. Data are presented as box-and-whiskers diagram with median and interquartile range. *P ≤ 0.05, **P ≤ 0.01, ***P < 0.001, one-way ANOVA
