Fig. 3
From: Three-dimensional scanless holographic optogenetics with temporal focusing (3D-SHOT)
3D-SHOT generates axially confined photo-activation. a A photostimulation pattern generated with CGH was mechanically stepped along the optical z-axis and passed through a cell expressing opsin. Photocurrents were recorded in the whole-cell voltage clamp configuration. b−f The response profile for CGH with a 10 µm disk target and different power levels on CHO cells for CGH b or 3D-SHOT f (n = 12 cells, data points are a representative example cell). d The FWHM of the characteristic response profile for both methods at various power levels on CHO cells (n = 12 cells, data represent mean ± s.e.m.). e As in a, but in mouse brain slices. c, g as in b, f, but in mouse brain slices (n = 11 neuron, data points are a representative example neuron). h The FWHM of the characteristic response profile for both methods at various power levels in mouse brain slices (n = 11 neurons, data represent mean ± s.e.m.)
