Fig. 7
From: Three-dimensional scanless holographic optogenetics with temporal focusing (3D-SHOT)
Power corrected 3D-SHOT simultaneously illuminates 50 spots. a Uncorrected simultaneous 3D-SHOT with a hologram targeting 50 spots in a spiral pattern, occupying 50 individual z-planes. Spots are color coded by normalized 2P absorption in each spot (measured with a sub-stage camera). b Correction factor computed for each spot via 3D power interpolant. c Measurements of 2P-induced fluorescence with 3D-SHOT, and a power-corrected hologram targeting the same locations as in a. d Mean intensity images of power-corrected 50 spot spiral hologram showing (z,y) (top left), (z,x) (bottom left), or (x,y) (right) projection views. e Box plots showing the variance in normalized 2P absorption from each spot within the corresponding hologram before and after power correction (p < 1 × 10−12, F-test of equality of variances, error bars correspond to range, red line is median, n = 50 spots)
