Fig. 4

Lmx1a/b regulate the expression of the axon guidance receptor Plxnc1. a Images of brain tissue section following quick TH staining, before LCM and in the tube cap after LCM. b In situ hybridization for Plxnc1 expression in ventral midbrain coronal sections of control and Lmx1a/b cKO mice at P1. c RT-qPCR analysis of Plxnc1 expression specifically in SNpc and VTA neurons isolated with LCM (SNpc: n = 3, two-tailed unpaired t test, p = 0.0353; VTA: n = 3 controls and 4 Lmx1a/b cKO, one-tailed Mann–Whitney U, p = 0.0286). d RT-qPCR quantification of Plxnc1 expression after Lmx1a and Lmx1b overexpression in ventral midbrain primary cell cultures at P1 (n = 3 independent cultures; for Plxnc1: one-way ANOVA with Tukey’s post test, *p < 0.05; for Lmx1a and Lmx1b: one-tailed Mann–Whitney U, p = 0.05). Scale bars: 200 µm