Table 1 Antibodies used for this study
Antibodies | Concentration | Origin | Catalog number | Reference | |
|---|---|---|---|---|---|
Primary antibodies | Rabbit anti-TH | 1/1000 (IHC) | Pel-FreezBiologicals | P40101-0 | |
Sheep anti-TH | 1/1000 (IHC) | Millipore | AB1542 | ||
Rat anti-DAT | 1/500 (IHC) | Millipore | MAB369 | ||
Sheep anti-Plxnc1 | 1/150 (IHC) | R&D systems | AF5375 | ||
Rabbit anti-Lmx1a | 1/1000 (IHC) | Millipore | AB10533 | ||
Guinea-pig anti-Lmx1b | 1/100 (IHC) | Dr. Carmen Birchmeier, Max Delbruck Center of Molecular Medicine, Berlin, Germany | |||
Goat anti-Otx2 | 1/200 (IHC) | R&D systems | AF1979 | ||
Mouse anti-Tyrosine tubuline | 1/3000 (IHC) | Sigma | T9028 | ||
Mouse anti-Actin | 1/1000 (IHC) 1/10,000 (WB) | Millipore | MAB1501 | ||
Chicken anti-GFP | 1/500 (IHC) | Aves Labs | GFP-1020 | ||
Rabbit anti-Sema7a | 1/500 (WB) | Abcam | ab23578 | ||
Secondary antibodies | Donkey anti-mouse Alexa-Fluor-680 | 1/5000 (IHC) | Jackson ImmunoResearch | 715-625-150 | |
Donkey anti-sheep Alexa-Fluor-680 | 1/5000 (IHC) | Jackson ImmunoResearch | 713-625-147 | ||
Donkey anti-rabbit Alexa-Fluor-790 | 1/5000 (IHC) | Jackson ImmunoResearch | 711-655-152 | ||
Donkey anti-mouse Alexa-Fluor-790 | 1/5000 (IHC) | Jackson ImmunoResearch | 715-655-150 | ||
Donkey Alexa-Fluor-488 | 1/200 (IHC) | Jackson ImmunoResearch | |||
Donkey Alexa-Fluor-555 | 1/200 (IHC) | Jackson ImmunoResearch | |||
Donkey Alexa-Fluor-647 | 1/200 (IHC) | Jackson ImmunoResearch | |||
HRP-conjugated Goat anti-mouse | 1/5000 (WB) | Life Technologies | G-21040 | ||
HRP-conjugated Goat anti-rabbit | 1/3000 (WB) | Cell Signaling Technology | 7074P2 |
