Fig. 3
Identification of a receptor for DAG. a DAG colocalizes with connective tissue growth factor, CTGF. FAM-DAG was intravenously injected in 9-month-old hAPP-J20 and WT mice, allowed to circulate for 30 minutes, after which mice were perfused, and the brains were fixed, sectioned and stained for FAM (green) and CTGF (red). The region shown is the hippocampus. Scale bars, 50 µm (20 µm for inset). Representative images are shown. b DAG binds to recombinant CTGF. FAM-DAG was incubated on ELISA plate coated with recombinant human CTGF at two different peptide concentrations of the FAM peptide alone (light gray bars), or in the presence of non-labeled DAG (0.6 mM) (dark gray bars). The control peptide did not show significant binding to CTGF under the same conditions (open bars). Non-labeled DAG inhibited the binding of FAM-DAG, demonstrating specificity of the CTGF binding
