Fig. 4

DAG homing to early stage hAPP-J20 mouse brain. a DAG localizes in the cerebrocortex and hippocampus of young (3–4 month old) hAPP-J20 mice after intravenous administration. FAM-DAG or control peptide were intravenously injected and allowed to circulate for 30 min. The mice were perfused, and brains were fixed, sectioned and stained for FAM (green). The signal from FAM was quantified using ImageJ and plotted in bar graph. The region shown is the cortex. b DAG co-localizes with CTGF and blood vessels in the hAPP-J20 mice. Sections from panel a were stained for FAM (green), CTGF (magenta), GFAP (red in first row), and CD31 (red in second and third row). The region shown is the cortex. Arrowheads point at DAG (FAM)-CTGF-CD31 co-localization. The blood vessels lack detectable Aβ accumulation (bottom row). Scale bar, 50 µm. **P < 0.01