Fig. 5
Aβ and CTGF expression in human brain, and DAG binding to human AD brain sections. a Sections from an AD patient brain were stained for Aβ (yellow) and counterstained with DAPI. Scale bar, 1 mm b Representative immunohistochemistry images of CTGF in brain sections of an AD patient and a control individual. The signal was quantified and plotted (bar diagram). Scale bar, 100 µm. *P < 0.05. c Human AD brain sections were incubated with either FAM-DAG (top row), control peptide (middle row) or anti-CTGF followed by FAM-DAG (bottom row), and stained for Aβ (red). Sections were counterstained with DAPI. Green signal is from native FAM fluorescence from peptide. Scale bar, 50 µm. Inset shows higher magnification of boxed area from panel c co-stained with GFAP antibody (red). Scale bar, 20 µm
