Fig. 2 | Nature Communications

Fig. 2

From: Genome-scale single-cell mechanical phenotyping reveals disease-related genes involved in mitotic rounding

Fig. 2

Analysis of subcellular localization of selected mitotic cell mechanics proteins. Immunofluorescence images of fixed HeLa interphase (left) and mitotic (right) cells expressing selected EGFP-tagged proteins, which were identified to result in decreased relative equilibrium rounding force when downregulated by RNAi. These proteins include ACTN4, CLIC5, ECT2, FAM134A, MYH9, DJ-1, ROCK2 and TMEM63B. Dashed boxes show the location of the EGFP-tagged protein at the cell cortex at higher magnification. Blue, DNA (DAPI); Green/grey, EGFP (goat anti-GFP antibody/Alexa-488); Red, tubulin (mouse anti-alpha-tubulin/Alexa-594). Other relevant proteins had been tagged with EGFP using BAC TransgeneOmics26 and are analyzed in Supplementary Fig. 4. Scale bars, 10 µm

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