Fig. 4
From: Replication fork reversal triggers fork degradation in BRCA2-defective cells
Stable RAD51 nucleofilaments are required not to form, but rather to protect reversed forks from nucleolytic degradation. a Control (BJ) or RAD51-T131P fibroblasts were labeled with CldU (red) and IdU (green), followed by treatment with 4 mM HU for 5 h and 50 μM mirin for 6 h, as indicated. A set of representative DNA fibers from each condition is shown. Ratios of IdU vs. CldU tracts are plotted. Track length analysis and statistics as in Fig. 3a. b EM-based assessment of the frequency of reversed replication forks isolated from BJ and T131P treated as indicated (4 mM HU for 5 h; 50 μM mirin for 6 h). In brackets, the total number of analyzed molecules. Results of two independent EM experiments are in Supplementary Table 7
