Fig. 3
mGluR5 and GluN1 are more confined within the synaptic compartment in Fmr1 KO neurons. (a) Representative surface distribution of mGluR5-QD (upper panel) and GluN1-QD (lower panel) in a 500-frame stack (each dot represents the detection of a single receptor during 50-ms acquisition time), revealing the synaptic site as a trapping zone (green). (b) Relative fractions of synaptic mGluR5-QD (left panel) and GluN1-QD (right panel) particles. These values are increased in Fmr1 KO neurons (mGluR5-QD: WT, 8.053 ± 0.504 %, n = 16 dendritic fields from 3 cultures; Fmr1 KO, 15.95 ± 0.685 %, n = 14 dendritic fields from 3 cultures; ***P < 0.001, t = 9.44, df = 28, unpaired Student’s t-test; GluN1-QD: WT, 8.318 ± 0.382 %, n = 63 dendritic fields from 6 cultures; Fmr1 KO, 10.02 ± 0.446 %, n = 51 dendritic fields from 6 cultures; **P < 0.01, t = 2.91, df = 112, unpaired Student’s t-test)
