Fig. 2
From: Fbxo4-mediated degradation of Fxr1 suppresses tumorigenesis in head and neck squamous cell carcinoma
Fbxo4 ubiquitylates Fxr1 both in vivo and in vitro. a Fbxo4 reconstitution reduces Fxr1 levels in Fbxo4−/− MEFs. Note, arrow indicates Fbxo4; MEFs harbour a non-specific band that migrates below bona fide Fbxo4. b MLN-4924 rescues Fxr1 levels in both Fbxo4 + / + and −/− MEFs. c Fbxo4-induced Fxr1 ubiquitylation is enhanced by GSK3β co-expression and suppressed by MLN-4924 treatment for 6 h. d WT but not ΔF Fbxo4 ubiquitylates Fxr1 in vivo. e In vitro assay illustrates Fxr1 is ubiquitylated by Fbxo4. f Cyclin D1 ubiquitylation is used as a control for in vitro assays. g WT and S12E Fbxo4 enhance ubiquitylation of Fxr1 in vivo. h E379A, E380A and I377M Fbxo4 mutants lose the ability to ubiquitylate Fxr1 in vivo
