Fig. 3

Genome-wide mapping of SP5 binding sites. a Representative example of SP1- and SP5-chromatin binding. A segment of chromosome 4 illustrates the highly selective nature of SP5 binding relative to the ubiquitous transcription factor SP1. PLK4 serves a representative example of SP1 and SP5 binding in untreated and Wnt3a-treated cells. b A majority of SP5 peaks overlap with SP1 binding sites. A Venn diagram illustrates that 475 SP5 peaks overlapped with SP1 peaks. SP1 bound thousands of targets throughout the genome, whereas SP5 bound a select number of targets, including unique sites and sites shared with SP1. The complete list of SP5 peaks is provided in Supplementary Data 2. c A majority of SP5 binding sites map near transcriptional start sites. The schematic of a generic gene structure provides color-coding for the pie charts. Genomic binding locations of SP5 are categorized as transcriptional start site (TSS, defined as 2 kb upstream and downstream of TSS), exon, intron, and intergenic. d Motif analysis of SP5 peaks. SP5 binding events were analyzed for overrepresented sequence motifs using MEME. (i) The most overrepresented motif for the 475 SP5 peaks with an overlapping SP1 peak closely matches the GC box. (ii) The most overrepresented motif for the 187 SP5 peaks lacking an overlapping SP1 peak diverges from the canonical GC box. (iii) The most overrepresented motif for SP1 binding summits matches the GC box. e Motif enrichment for SP1 and SP5 binding events. The fractions of peaks matching the motif in d (i) are provided for all SP1 peaks (SP1 All), SP5 peaks with overlapping SP1 peaks (SP5 w/ SP1), all SP5 peaks (SP5 All), and SP5 peaks lacking an overlapping SP1 peak (SP5 w/o SP1). f SP5 and SP1 compete for binding at select sites. The normalized average peak score is provided for all SP1 peaks (SP1 peaks All), SP1 peaks that overlap with SP5 peaks (SP1 peaks with SP5 peaks) and for all SP5 peaks (SP5 peaks) from cells that were untreated or Wnt3a-treated