Fig. 3
From: The circular RNA circBIRC6 participates in the molecular circuitry controlling human pluripotency
C ircBIRC6 or circCORO1C promote hESC pluripotency. a Schematic illustrating the major components of circRNA minigene constructs. Arrows indicate the placement and direction of RT-qPCR primers used to amplify circRNA or precursor transcripts. b–d RT-qPCR analysis of the expression of b circRNAs and their precursor transcripts, and the indicated c pluripotency-associated genes and d lineage-associated genes in hESCs transfected with a circRNA minigene (H9-circB, H9-circC, and H9-circMa) cultured under differentiation conditions. e ICC analysis and f quantitative analysis of the expression of the pluripotency and lineage markers as indicated in hESCs transfected with a circRNA minigene (H9-circB, H9-circC, and H9-circMa) and cultured under differentiation conditions. Nuclei were stained with DAPI (blue). Scale bar: 20 μm. Quantitative data from three independent experiments is presented as mean ± SD (error bars). P-values were determined by two-tailed two-sample t-tests (*P < 0.05; **P < 0.01; ***P < 0.001)
