Fig. 2

Summary of SUMOylation site identifications from this study and comparison with previous studies. a Venn diagram showing overlap between the 1209 SUMO sites identified in this study from both Hela and HCT116 cell lines (blue) and all SUMO modifications from either Hendriks et al. (red) or Phosphosite Plus (yellow). b Identified spectrum of a novel SUMO modification site identified in DNA-PK that would have resulted in a 57-amino-acid tryptic peptide. The sequence above the spectrum shows the tryptic cleavage sites in blue, the modification site is in red, and the matched sequence from the WaLP digest is underlined. c Identified spectrum of a previously uncharacterized SUMO modification site in Sp100 that would result in only a six-residue tryptic peptide