Fig. 1
From: High reactivity of deep biota under anthropogenic CO2 injection into basalt
Contrasting temporal patterns in groundwater of monitoring well HN-04 and control well HN-01. pH (a) and conductivity (b) measured in the field, number of operational taxonomic units (OTUs) (c) and Shannon index (d), both obtained in duplicates from 454-pyrosequencing of 16S-rRNA amplicons (Supplementary Fig. 5). Also shown is gene abundance obtained using quantitative polymerase chain reaction (qPCR) for bacteria (e) and Betaproteobacteria (f). Betaproteobacteria taxonomy established from pyrotag sequencing is also indicated in f. The yellow boxes frame the pre-injection measurements, whereas the gray box indicates the time period displaying the highest dissolved inorganic carbon (DIC) concentration at the level of well HN-04 (March 2012) that resulted in groundwater acidification shown in a. Errors (i.e., standard deviation) on analytical measurement for pH and conductivity are smaller than symbols. For details on the injection site and geochemical monitoring, see Supplementary Figs. 1 and 2, respectively, along with Supplementary Tables 1 to 3
