Fig. 3
Shelterincore complex bound at ss/ds telomeric junction remains stable in the presence of excess telomeric single-stranded or double-stranded DNA. a Fluorescence polarization assay to measure the dissociation rate of pre-formed shelterincore-Alexa488-C8 probe protein–DNA complex in the presence of excess single-stranded, double-stranded and ss/ds junction telomeric competitors. 5 nM of Alexa488-C8 probe was incubated with 100 nM of shelterincore complex for 30 min before adding 1 µM competitor (200-fold excess to fluorescent probe). b Comparison of the complex dissociation rate with single-stranded telomeric competitors with either 3′ TTAGGG or TTAG. c Comparison of dissociation rates of complexes with 1 and 10 µM single-stranded telomeric DNA competitor. d Summary of complex dissociation rates in the presence of various telomeric competitors obtained from single-exponential decay function fitting. (Error bars are s.d., n = 3 independent experiments using 2 different protein batches.)
