Fig. 4 | Nature Communications

Fig. 4

From: Reconstitution of human shelterin complexes reveals unexpected stoichiometry and dual pathways to enhance telomerase processivity

Fig. 4

Human shelterin complexes have a 2:1 TRF2:TIN2 stoichiometry. a Size-exclusion chromatography multi-angle light scattering (SEC-MALS) assay to determine absolute molecular mass of various purified human shelterin complexes. b Summary of the shelterin complexes molecular mass (percentage error from software fitting) and the expected molecular stoichiometry. c Shelterin complexes were expressed with a single ybbR tag for each subunit. d The purified complexes were labeled with fluorescent dye (Cy3) for relative stoichiometry analysis in a SDS-PAGE gel by band fluorescence-intensity measurement. e The quantified fluorescence intensities were normalized to TPP1 counts and compared. (Error bars represent the range of data, n = 2 independent experiments using different protein batches.)

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