Fig. 7
Phosphorylation of Ser1116 containing the Leu1120/Leu1121 (pSVRPLL) motif is sufficient for endocytosis. a Schematic representation of chimeric protein. The plasma membrane targeting signal of Lyn and EGFP was fused to the C-terminal cytoplasmic region (CT) of ATP11C. b Amino acid sequences in the C-terminal cytoplasmic region (CT) of human ATP11C, which were fused to Lyn-EGFP as shown in a. c HeLa cells were transiently transfected with expression vector for Lyn-EGFP-tagged CT-WT, CT-9-Ala, CT-8-Ala/S1116, or CT-8-Ala/S1126, or the original vector encoding Lyn-EGFP-tagged FRB (FKBP–rapamycin-binding domain). The cells were treated for 15 min with vehicle alone (Mock), 400 nM PMA (PMA), or 1 μM A23187 in the presence of 1.8 mM CaCl2 (A23187), followed by fixation. Scale bars, 20 μm
