Fig. 4

WGCN analysis identifies Prkag2 in a central hub of pacemaker regulating genes. a Weighted gene co-expression network (WGCN) analysis-derived visualization of the iSAB–aCaB gene network by heat map plot. The heat map shows the topological overlap matrix (TOM) among all genes in the analysis. Light yellow represents low overlap and darker red represents higher overlap. Blocks along the diagonal are modules. Dendrograms and module color assignments are shown at the top and along the left side, respectively. b Refinement of the gene modules showing the gene dendrogram (average linkage) and module color assignment based on dynamic hybrid TOM clustering. c Plot of gene significance and intra-modular connectivity illustrating high correlation within the green module containing Prkag2. d Plot of co-expressed genes in the green module vs gene significance subdividing iSABs and aCaBs. e Further investigation of the relationship and connectivity among the investigated modules illustrated by (upper portion) a hierarchical clustering dendrogram (average linkage) and (lower portion) eigenvalue adjacency heatmap. f Multi-dimensional scaling plot identifying the green module as a major signaling hub connecting multiple genes critical to pacemaker functionality. g Heat map illustrating the TOM among genes depicted in e. Each column and row refers to a single gene. Light yellow represents low overlap and darker red represents higher overlap