Fig. 5
Pharmacological activation of AMPK reduces the spontaneous beating rate of iSABs. a ELISA analysis of α AMPK Thr172 phosphorylation in iSABs treated with the AMPK activator AICAR- or the small-molecule AMPK activator compound 991 (n = 4). b Effect of incubation with variable doses of AICAR or control on iSAB-beating rate. c Effect of incubation with variable doses of compound 991 or control on iSAB-beating rate. d Bar chart representation of AICAR dose–response effect data shown in b specifically for the 48 h incubation time point. e Bar chart representation of compound 991 dose–response effect data shown in c specifically for the 48 h incubation time point. a Two-way ANOVA was performed. d, e One-way ANOVA followed by Holm–Sidak’s multiple comparisons test was performed. *P < 0.05, **P < 0.01,***P < 0.001, ****P < 0.0001. a–e Data are shown as means ± s.e.m.
