Fig. 6
Adenovirus-mediated γ2 AMPK gain-of-function reduces intrinsic firing rate of WT mammalian SA cells. a Mean firing rates of SA cells isolated from WT C57BL/6J mice following infection with adenoviruses carrying control (Ad-mCherry), WT γ2 (Ad-WT γ2), or R299Q γ2 (Ad-R299Q γ2) constructs (n = 7–9). b Mean spontaneous beating rate of WT rabbit SA cells following adenoviral infection (n = 29–36). c Representative line-scan images of spontaneous contraction (column 1), scan line (column 2), mCherry density (column 3), and corresponding transmission images (column 4) of WT rabbit SA cells following adenoviral infection. d Mean firing rates of SA cells isolated from homozygous R299Q γ2 mice following adenoviral infection with control (Ad-mCherry) or WT γ2 constructs (n = 5–9). Mean firing rate of WT SA cells following infection with Ad-mCherry (bar identical to that in a) also depicted for comparison. a, b, d One-way ANOVA followed by Holm–Sidak’s multiple comparisons test was performed. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; ns not significant. a, b, d Data are shown as means ± s.e.m
