Fig. 5

Analysis of in nucleo acylation. a Schematic representation of in nucleo acylation assay. b Bar plots showing the dose-dependent acylation of histones upon treatment with 0, 1 and 5 μM of acetyl-, butyryl-, malonyl-, glutaryl-, propionyl-, succinyl-, β-hydroxybutyryl and crotonyl-CoA, respectively. Values represent the sum of the relative abundances of all acylated peptides from histones H3 and H4. c Example of in nucleo acylation resulting in an increase in malonylation of H3K56 after treatment with increasing concentrations of malonyl-CoA. d Example of in nucleo acylation showing that induced propionylation by increasing the concentration of propionyl-CoA resulted into a reduced relative abundance of acetylation on the site H3K14